Ultra Sensitive Visible Spectroscopic Methods for Cefpodoxime Proxetil Using NQS and PDAC Reagents

Very sensitive and accurate visible spectrophotometric methods were developed and validated to quantify Cefpodoxime Proxetil in API and marketed formulation. Method A is based on the measurement of absorbance of a reddish-orange coloured chromogen at 454.9 nm, formed by the condensation reaction of the primary amino group of Cefpodoxime Proxetil with NQS reagent. Method B is based on the measurement of absorbance of a light yellowish green coloured chromogen at 401.3 nm, due to the Schiff’s base formed between Cefpodoxime Proxetil and PDAC reagent in acidic medium. Beer-lambert’s law is obeyed in the concentration range of 0.1-1µg/mL for method A, and for method B, it was 1-10 µg/mL. The values of LOD and LOQ were found to be 0.009 µg/mL and 0.027 µg/mL, respectively, for the condensation reaction with NQS reagent. For Schiff’s base reaction, LOD and LOQ values were 0.154 µg/mL and 0.467 µg/mL respectively. The results of method validation done in accordance with ICH guidelines were satisfactory. The developed method can be successfully employed in routine analysis of Cefpodoxime Proxetil in pharmaceutical dosage forms.